1 Inject the collected fat into the Inner Chamber.
(The injection method may vary depending on the equipment.)
2Inject enzyme solution. (Same as step 1)
3After enzyme treatment (40 mins to 1 hr), perform centrifugation(3 mins).
4Remove the enzyme and fat mixture in the center of the chamber.
5Inject the washing solution. (Same as steps 1 and 2.)
6Remove the washing solution in the center of the chamber after the washing and centrifugation process.
7Repeat the washing process 2-3 times.
8The washed and separated stem cells are collected in both collection chambers (wings).
9Collect the separated stem cells using a syringe through the green needle injection port.
The SVF that passed through the 100um filter can be immediately used for IV injection.