Advantages
01
Use independent storage tubes to avoid cross-contamination.
02
Rack Index that can be checked immediately without taking out the sample.
03
Stable maintenance of temperature optimized for tissue and cell storage using self-developed triple Cryo Tube.
04
Minimize damage to stored samples by minimizing temperature changes that occur during the process of inserting and removing samples.
05
Refrigeration, light, freezing, and deep freezing can be used simultaneously in one CryoHybrid unit.
06
More than 2,300 stored samples.
07
Reduces maintenance costs by not using liquid nitrogen.
08
Minimize sample damage by preventing ice crystallization through pre-freezing port.
09
Equipped with a temperature recording device to automatically record temperature changes within the equipment.
10
Equipped with an uninterruptible power supply to safely store samples even in emergency situations such as power outages.
3 Storage Locations Divided by Function
1Refrigerated storage locationRefrigerated sample storage
2Weak FreezingCryo-vial(+CPA) storage
3Storng Freezing
Independent storage system : Prevent cross-contamination by using individual storage containers
A minimum of 2,300 Cryo-vial quantites can be stored in stock : Stores 12 vials in 1 CryoHybrid Tube
Storage Process - How to Use
1Take out the Cryo-vial(+CPA) from the weak freezer position.
2Move the frozen Cryo-vial(+CPA) to a refrigeratied location and mix it well with the prepared sample when the CPA has melted.
3Insert the prepared Cryo-vial into the transparent tube in the CryoHybrid Tube.
4Store at the strong freezing mode.
Components
Protocol - Washing for Cryopreserved SVF
1![protocol](/rsc/front/images/common/protocol_imgB1.jpg)
Remove the marked individual Cryo-Vial from the storage rack and place in the refrigerated location to thaw the sample in the Cryo-Vial.
2![protocol](/rsc/front/images/common/protocol_imgB2.jpg)
Open the Cryo-Vial lid in the storage stand and extract it with a syringe.
3![protocol](/rsc/front/images/common/protocol_imgB3.jpg)
Inject SVF thawed in a CryoHybrid into the Inner-Chamber containing the culture chips.
4![protocol](/rsc/front/images/common/protocol_imgB4.jpg)
Inject culture solution.
5![protocol](/rsc/front/images/common/protocol_imgB5.jpg)
Proceed washing in forward and reverse directions (left) so that the contents mix well.
6![protocol](/rsc/front/images/common/protocol_imgB6.jpg)
Rroceed washing in forward and reverse directions (right).
7![protocol](/rsc/front/images/common/protocol_imgB7.jpg)
Proceed centrifugation.
8![protocol](/rsc/front/images/common/protocol_imgB8.jpg)
Remove the washing solution.
9![protocol](/rsc/front/images/common/protocol_imgB9.jpg)
Reinject the culture solution.
10![protocol](/rsc/front/images/common/protocol_imgB10.jpg)
Proceed conditioning culture for 48 hours.
11![protocol](/rsc/front/images/common/protocol_imgB11.jpg)
Proceed centrifugation.
12![protocol](/rsc/front/images/common/protocol_imgB12.jpg)
Extract tje SVF gathered at both ends.